PROLIFERATION AND APOPTOSIS OF B220(-)CD8(-)TCR-ALPHA-BETA(INTERMEDIATE) T-CELLS IN THE LIVER OF NORMAL ADULT MICE - IMPLICATION FOR LPR PATHOGENESIS()CD4()

Small numbers of T cells have been isolated from the normal mouse live r and many of these are of the CD4(-)CD8(-)TCR alpha beta(+) phenotype . Larger numbers of such cells are present in the livers of mice homoz ygous for the lpr mutation and the liver has been proposed to be the s ite of an extrathymic T cell development pathway that is expanded in l pr/lpr mice. Using a modified separation procedure that increases the liver T cell yield, we have been able to characterize a subset of CD4( -)CD8(-)TCR alpha beta(intermediate) T cells that express the B220 epi tope of the CD45 molecule, and resemble in this and many other ways th e accumulating T cells in lpr lymph nodes. These cells are an actively dividing population and even in healthy, unmanipulated mice a large p roportion of them are undergoing apoptosis. We propose the model that the normal liver is a major site for T cell destruction and that the l pr defect results in failure of this process with leakage of B220(+)CD 4(-)CD8(-)TCR alpha beta(+) cells from the liver to peripheral lymphoi d tissues, particularly lymph nodes.