RESOLUTION OF EMBEDDED CHROMATOGRAPHIC PEAKS BY SEQUENTIAL RANK ANALYSIS OF THE 1ST-ORDER DIFFERENTIATED ELUTION PROFILE IN THE TIME-DOMAIN

Assuming local symmetry around chromatographic peak maxima of the indi vidual analytes, a new procedure is developed for identification of su ch maxima in multiwavelength chromatograms with embedded peaks. At the se maxima the spectroscopic profiles of the pure analytes are directly available from the first-order differentiated elution profile in the time domain. When the chromatographic profiles of the pure analytes ar e not symmetric, for instance due to tailing of peaks, the identificat ion of the maxima still appears to be possible by means of latent-proj ective graphs. The quality of the results can be checked by comparison with partial resolution results from other procedures, such as heuris tic evolving latent projections (HELP) or orthogonal projections. The approach is investigated on some simulated systems.