INTERFERENCE OF THE PHENYLPYRROLE FUNGICIDE FENPICLONIL WITH MEMBRANES AND MEMBRANE-FUNCTION

Citation
Abk. Jespers et al., INTERFERENCE OF THE PHENYLPYRROLE FUNGICIDE FENPICLONIL WITH MEMBRANES AND MEMBRANE-FUNCTION, Pesticide science, 40(2), 1994, pp. 133-140
Citations number
53
Categorie Soggetti
Agriculture
Journal title
ISSN journal
0031613X
Volume
40
Issue
2
Year of publication
1994
Pages
133 - 140
Database
ISI
SICI code
0031-613X(1994)40:2<133:IOTPFF>2.0.ZU;2-1
Abstract
The phenylpyrrole fungicide fenpiclonil accumulates to high levels in mycelium of Fusarium sulphureum and artificial liposomes. The accumula tion is a result of a physicochemical distribution of the fungicide ov er lipid-like material of the mycelium and the medium. Accumulation is reversible, as the fungicide can easily be removed from the mycelium by washing with water. Fenpicionil is not metabolized by the fungus du ring incubation for 24 h. The fungicide affects neither membrane fluid ity in artificial liposomes nor amino acid accumulation in bacterial v esicles. Thus, accumulation of the fungicide does not seem to affect t he functioning of membranes. Fenpiclonil induces the accumulation of t he membrane-potential probe tetraphenylphosphonium bromide by F. sulph ureum. Since accumulation of this probe probably reflects the cumulati ve potential over all cell membranes, the increased accumulation is mo re likely a result of changes of potentials over membranes of intracel lular organelles rather than plasma membrane hyperpolarization. Hence, the previously described effects of fenpiclonil on amino acid and sac charide uptake cannot be explained by plasma membrane hyperpolarizatio n.