ONE-STEP ISOLATION OF A CHITINASE BY AFFINITY-CHROMATOGRAPHY OF THE CHITINOLYTIC ENZYME COMPLEX PRODUCED BY STREPTOMYCES-KURSSANOVII

Authors
ILYINA AV VARLAMOV VP TIKHONOV VE YAMSKOV IA DAVANKOV VA
Citation
Av. Ilyina et al., ONE-STEP ISOLATION OF A CHITINASE BY AFFINITY-CHROMATOGRAPHY OF THE CHITINOLYTIC ENZYME COMPLEX PRODUCED BY STREPTOMYCES-KURSSANOVII, Biotechnology and applied biochemistry, 19, 1994, pp. 199-207
Citations number
14
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biotechnology and applied biochemistry → ACNP
ISSN journal
08854513
Volume
19
Year of publication
1994
Part
2
Pages
199 - 207
Database
ISI
SICI code
0885-4513(1994)19:<199:OIOACB>2.0.ZU;2-A
Abstract
One of the four chitinases with a molecular mass of 42 kDa existing in the chitinolytic enzyme complex produced by Streptomyces kurssanovii was separated in homogeneous form using one-step affinity chromatograp hy on cross-linked and phosphorylated chitin-type sorbents. Three othe r chitinases were not selectively bound by the sorbents and were not o btained in homegeneous form. The affinity sorbents were shown to be st able over the time of separation and could be used repeatedly.