GENE-EXPRESSION OF A THERMOSTABLE BETA-GALACTOSIDASE IN MAMMALIAN-CELLS AND ITS APPLICATION IN ASSAYS OF EUKARYOTIC PROMOTER ACTIVITY

Authors
CANNIO R DEPASCALE D ROSSI M BARTOLUCCI S
Citation
R. Cannio et al., GENE-EXPRESSION OF A THERMOSTABLE BETA-GALACTOSIDASE IN MAMMALIAN-CELLS AND ITS APPLICATION IN ASSAYS OF EUKARYOTIC PROMOTER ACTIVITY, Biotechnology and applied biochemistry, 19, 1994, pp. 233-242
Citations number
35
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biotechnology and applied biochemistry → ACNP
ISSN journal
08854513
Volume
19
Year of publication
1994
Part
2
Pages
233 - 242
Database
ISI
SICI code
0885-4513(1994)19:<233:GOATBI>2.0.ZU;2-Y
Abstract
The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cl oned and expressed in simian CV1 and murine NIH3T3 cell lines. The rec ombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as the wild-type Ss beta-gal and has no cytotoxic effect on the host cells. Its possible use as a repor ter gene is also proposed, and a comparison with other reporter gene s ystems is made.