CONTROL OF GROWTH AND RECOMBINANT PROTEIN-SYNTHESIS BY HEAT-SHOCK - IN A MUTANT MAMMALIAN-CELL LINE

Authors
HOVEY A BEBBINGTON CR JENKINS N
Citation
A. Hovey et al., CONTROL OF GROWTH AND RECOMBINANT PROTEIN-SYNTHESIS BY HEAT-SHOCK - IN A MUTANT MAMMALIAN-CELL LINE, Biotechnology letters, 16(3), 1994, pp. 215-220
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
3
Year of publication
1994
Pages
215 - 220
Database
ISI
SICI code
0141-5492(1994)16:3<215:COGARP>2.0.ZU;2-0
Abstract
The effect of heat-shock on cell growth and the induction of recombina nt protein synthesis by a temperature-sensitive (ts) Chinese hamster o vary (CHO) cell line was investigated. An optimal regime of successive 2 hour heat-shocks (42 degrees C) over 72 hours was found to simultan eously arrest cell growth and induce the synthesis of recombinant prot ein. The enhanced induction achieved from growth arrested cells may fi nd application in the production of cytotoxic proteins.