A. Hovey et al., CONTROL OF GROWTH AND RECOMBINANT PROTEIN-SYNTHESIS BY HEAT-SHOCK - IN A MUTANT MAMMALIAN-CELL LINE, Biotechnology letters, 16(3), 1994, pp. 215-220
The effect of heat-shock on cell growth and the induction of recombina
nt protein synthesis by a temperature-sensitive (ts) Chinese hamster o
vary (CHO) cell line was investigated. An optimal regime of successive
2 hour heat-shocks (42 degrees C) over 72 hours was found to simultan
eously arrest cell growth and induce the synthesis of recombinant prot
ein. The enhanced induction achieved from growth arrested cells may fi
nd application in the production of cytotoxic proteins.