INVESTIGATION OF APIGENIN-7-0-BETA-GLUCOSIDE HYDROLYSIS BY BETA-GLUCOSIDASE FROM ALMONDS

Authors
PEKIC B ZEKOVIC Z LEPOJEVIC Z
Citation
B. Pekic et al., INVESTIGATION OF APIGENIN-7-0-BETA-GLUCOSIDE HYDROLYSIS BY BETA-GLUCOSIDASE FROM ALMONDS, Biotechnology letters, 16(3), 1994, pp. 229-234
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
3
Year of publication
1994
Pages
229 - 234
Database
ISI
SICI code
0141-5492(1994)16:3<229:IOAHBB>2.0.ZU;2-U
Abstract
The enzymatic transformation of apigenin-7-O-beta-glucoside into apige nin is effected using beta-glucosidase from almonds. Kinetic studies g ave the following values K-m=7.63x10(-4)mol/dm(3) -1 -1 m v(max)=10.37 mu mol mg(-1) min(-1) and E(1)=65.81 kJ/mol when the substrate was pu re apigenin-7-O-beta-glucoside, while in case of apigenin-7-O-beta glu coside from dry extract of camomile ligulate flowers E(2)=65.57 kJ/mol .