H. Razafindralambo et al., IMPROVEMENT OF ENZYMATIC-SYNTHESIS YIELDS OF FLAVOR ACETATES - THE EXAMPLE OF THE ISOAMYL ACETATE, Biotechnology letters, 16(3), 1994, pp. 247-250
Isoamyl acetate synthesis was chosen as a model to improve flavour ace
tate yields by optimising the enzymatic reaction. Alcohol:acid molar r
atio, temperature, water content and amount of enzyme effects were ana
lyzed. The optimum values were respectively 4, 45 degrees C, 0,1% (w/v
) and 0,5 g. In these conditions, the synthesis yield reached 80% afte
r 24 h of reaction and was found 15 times greater than those already r
eported in the literature.