SEPARATION OF SESQUITERPENE LACTONES FROM ARNICAE-FLOS DAB-10 BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Capillary electrophoresis in the micellar mode offers a further analyt ical method to identify the sesquiterpene lactones in Arnicae dos DAB 10 originating from Arnica montana and A, chamissonis subsp, foliosa. Compared to high performance liquid chromatographic analysis on revers ed-phase columns, nearly the same separation of these active compounds may be obtained, Polar alcohols are eluted before esterified derivati ves, and the sesquiterpene lactones, derived from 11 alpha,13-dihydroh elenalin, eluted before the corresponding compounds with an exomethyle ne group at C-11, Furthermore, the elution order is influenced by subs titution at C-2 and C-6 of the skeleton. (C) 1997 by John Wiley & Sons , Ltd.