Ej. Threlfall et al., INSERTION-SEQUENCE IS200 FINGERPRINTING OF SALMONELLA-TYPHI - AN ASSESSMENT OF EPIDEMIOLOGIC APPLICABILITY, Epidemiology and infection, 112(2), 1994, pp. 253-261
When Pst I-generated digests of genomic DNA from each of the type stra
ins of 49 of the Vi phage types of Salmonella typhi were probed with a
PCR-amplified IS200 gene probe, all strains were found to possess at
least 11 IS200 elements carried on fragments in the range 24.2-1.2 kb.
Fourteen fingerprints were identified but two patterns designated IS2
00Sty1. and IS200Sty2 predominated. In one strain, a. plasmid-mediated
IS200 element was identified. When IS200 fingerprinting was applied t
o epidemiologically-unrelated strains of S. typhi isolated in Ecuador,
3 patterns were identified in 10 strains belonging to 9 different pha
ge types. It is concluded that Vi phage typing remains the method of c
hoice for the primary differentiation of X. typhi but that IS200 finge
rprinting way be of limited use in laboratories which do not have acce
ss to phage typing.