DEVELOPMENT, APPLICATION AND COMPARISON OF AN ENZYME-IMMUNOASSAY AND A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF THE AROMATASE INHIBITOR CGS-20-267 IN BIOLOGICAL-FLUIDS
Cu. Pfister et al., DEVELOPMENT, APPLICATION AND COMPARISON OF AN ENZYME-IMMUNOASSAY AND A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF THE AROMATASE INHIBITOR CGS-20-267 IN BIOLOGICAL-FLUIDS, Journal of pharmaceutical sciences, 83(4), 1994, pp. 520-524
CGS 20 267 is a new potent and selective, nonsteroidal, oral aromatase
inhibitor. For its determination in human plasma and urine, an enzyme
immunoassay (EIA) and an HPLC method were developed. The EIA showed g
ood precision and accuracy (intra- and interassay variation between 3.
0 and 17.7%, recoveries between 81 and 106%) and a quantitation limit
of 0.7 nmol/L. A strong cross reactivity of the antibodies with the hy
droxy metabolite of CGS 20 267 (CGP 44 645) was observed. The HPLC met
hod showed a quantitation limit in plasma of 28 and 34 nmol/L for CGS
20 267 and CGP 44 645, respectively. For urine, concentrations down to
180 nmol/L (CGS 20 267) and 210 nmol/L (CGP 44 645) could be measured
. A cross check between EIA and HPLC on plasma samples from healthy ma
le volunteers or breast cancer patients treated orally with CGS 20 267
revealed an excellent correlation (slope = 0.934, intercept = 26, r =
0.991). However, the EIA measurements of urine samples yielded 3-25 t
imes higher concentrations than those obtained by HPLC. Further, HPLC
analysis revealed the presence of CGS 20 267 and crossreacting metabol
ites in urine but not in plasma. Therefore, the EIA can only be used f
or the determination of CGS 20 267 in plasma samples.