I. Levin et al., MAPPING DNA POLYMORPHISMS USING PCR PRIMERS DERIVED FROM THE SEQUENCEOF AN AVIAN CR-1 ELEMENT, The Journal of heredity, 85(2), 1994, pp. 73-78
Primers complementary to the chicken middle repetitive sequence elemen
t CR1 were used to generate and simultaneously map polymorphic polymer
ase chain reaction products (CR1-PCR) with various chicken DNAs as tem
plates. Ten primers were prepared using the sequence of a single CR1 e
lement as a guide. These 10 primers generated 23 polymorphic CR1-PCR p
roducts. The average number of polymorphic CR1-PCP products generated
using single primers (1.1 per primer) was significantly higher than th
e average number observed using combinations of two primers (0.3 per p
rimer combination). The polymorphic CR1-PCR products were mapped in a
subset of a reference backcross population designed for the genetic li
nkage analysis of the chicken. Nineteen of the polymorphic CR1-PCR pro
ducts identified were assigned to 13 of 19 linkage groups characterize
d thus far in this population; three have yet to be linked to a specif
ic map location. One of the CR1-PCR markers mapped to the chicken Z ch
romosome. There was no evidence for a significant clustering of CR1-PC
R markers within the map, even at the site of the CR1 element whose se
quence was used for primer design.