EXTRACTION, PARTIAL-PURIFICATION AND CHARACTERIZATION OF A BACTERIOCIN (FRAGILICIN) PRODUCED BY A STRAIN OF BACTEROIDES-FRAGILIS ISOLATED FROM CALLITHRIX PENICILLATA

Authors
FARIAS LM TOTOLA AH MIRANDA CMS CARVALHO MAR DAMASCENO CAV TAVARES CAP CISALPINO EO VIEIRA EC
Citation
Lm. Farias et al., EXTRACTION, PARTIAL-PURIFICATION AND CHARACTERIZATION OF A BACTERIOCIN (FRAGILICIN) PRODUCED BY A STRAIN OF BACTEROIDES-FRAGILIS ISOLATED FROM CALLITHRIX PENICILLATA, Research in microbiology, 145(1), 1994, pp. 9-16
Citations number
28
Categorie Soggetti
Microbiology
Journal title
Research in microbiology → ACNP
ISSN journal
09232508
Volume
145
Issue
1
Year of publication
1994
Pages
9 - 16
Database
ISI
SICI code
0923-2508(1994)145:1<9:EPACOA>2.0.ZU;2-7
Abstract
A strain of Bacteroides fragilis, isolated from the marmoset Callithri x penicillata, produced protein(s) with bacteriocin activity (fragilic in). Two active fractions (36 and 150 kDa) were isolated by chromatogr aphy. The bacteriocin exhibited iso- and hetero-antagonism. It remaine d stable between pH 3 and 10 and at 60 degrees C for 24 h. Pronase, tr ypsin, proteinase K and type VII protease inactivated the bacteriocin, giving evidence of its protein nature.