DETECTION OF ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES (ANCA) USING THE IMMUNOPEROXIDASE METHOD

Anti-neutrophil cytoplasmic antibodies (ANCA), which are present in We gener's granulomatosis (WG) and other systemic vasculitis, were detect ed using the immunoperoxidase method rather than the indirect immunofl uorescence method. It was possible to distinguish ANCA patterns (c-ANC A, p-ANCA) by the immunoperoxidase method. When neutropil smears were treated with 0.4% hydrogen peroxide solution, the endogenous peroxidas e activity was sufficiently inhibited. ANCA was also detected, using H L-60 cells (human promyelocytic leukemia) and THP-1 cells (human myelo cytic leukemia) as substrates.