H. Toro et al., LACRIMAL ANTIBODY-RESPONSE OF SPECIFIC PATHOGEN-FREE CHICKENS AND CHICKENS WITH MATERNAL IMMUNITY AFTER INFECTIOUS-BRONCHITIS VIRUS VACCINATION, Preventive veterinary medicine, 18(4), 1994, pp. 267-274
Lachrymal fluid specific IgG and IgA were detected by ELISA in chicken
s with specific maternal antibodies and in chickens free of antibodies
(SPF), after vaccination at 1 day of age with the H-120 vaccine strai
n of infectious bronchitis virus. Samples were obtained at 3 day inter
vals and until Day 37 of age. Optical densities obtained were in all i
nstances low but significant differences could be detected within and
between the experimental groups. Both class-specific immunoglobulins s
howed a similar kinetic pattern. Nevertheless, the SPF group increased
its IgA level on Day 13 while chickens with maternal immunity increas
ed their level on Day 16. The antibody levels of both IgG and IgA were
also different, being higher in the SPF group. In both chicken groups
, higher levels of IgA than IgG were detected.