Kr. Hinga et al., CARBON-ISOTOPE FRACTIONATION BY MARINE-PHYTOPLANKTON IN CULTURE - THEEFFECTS OF CO2 CONCENTRATION, PH, TEMPERATURE, AND SPECIES, Global biogeochemical cycles, 8(1), 1994, pp. 91-102
Closed cultures of marine phytoplankton were established under variabl
e conditions of CO2 concentration, temperature, growth rate (by light
limitation), and pH (hut with nearly identical [CO2aq]) in order to as
sess the relative influence of these variables on the extent of carbon
isotope fractionation relative to dissolved inorganic carbon sources.
Culture biomass was not allowed to increase beyond levels that would
significantly affect the dissolved carbon system in the closed culture
s. In experiments with Skeletonema costatum and Emiliania huxleyi, inc
reasing CO2 concentrations led to increased carbon isotope discriminat
ion (resulting in organic matter progressively depleted in deltaC-13C,
i.e., a greater, more negative epsilon(p)). Epsilon(p) values for E.
huxleyi were 8-10 parts per thousand less than for S. costatum under i
dentical conditions. For the S. costatum cultures, there was nearly a
20%. range in [CO2aq]-dependent epsilon(p). The effect was nonlinear w
ith a leveling off at high [CO2aq]. Over a pH range of 7.5-8.3 but at
a constant [CO2aq] there was a variation in carbon isotope fractionati
on by S. costatum of about 9 parts per thousand with a minimum at pH 7
.8-7.9. There was a temperature effect of -8 parts per thousand on fra
ctionation even after equilibrium temperature dependency of deltaC-13
of CO2aq was taken into account. No growth rate effect was found for S
. costatum over a modest range of growth rates. Culture experiments us
ed to determine the carbon isotope fractionation by phytoplankton spec
ies must be conducted under well-defined conditions of temperature, pH
, and CO2 concentrations. Hindcasts of ancient atmospheric pCO2 from m
easurements of deltaC-13 of organic carbon in marine sediments will re
quire careful calibration because of the variety of possible factors t
hat influence deltaC-13org.