CARBON-ISOTOPE FRACTIONATION BY MARINE-PHYTOPLANKTON IN CULTURE - THEEFFECTS OF CO2 CONCENTRATION, PH, TEMPERATURE, AND SPECIES

Citation
Kr. Hinga et al., CARBON-ISOTOPE FRACTIONATION BY MARINE-PHYTOPLANKTON IN CULTURE - THEEFFECTS OF CO2 CONCENTRATION, PH, TEMPERATURE, AND SPECIES, Global biogeochemical cycles, 8(1), 1994, pp. 91-102
Citations number
49
Categorie Soggetti
Metereology & Atmospheric Sciences","Geosciences, Interdisciplinary","Environmental Sciences
ISSN journal
08866236
Volume
8
Issue
1
Year of publication
1994
Pages
91 - 102
Database
ISI
SICI code
0886-6236(1994)8:1<91:CFBMIC>2.0.ZU;2-H
Abstract
Closed cultures of marine phytoplankton were established under variabl e conditions of CO2 concentration, temperature, growth rate (by light limitation), and pH (hut with nearly identical [CO2aq]) in order to as sess the relative influence of these variables on the extent of carbon isotope fractionation relative to dissolved inorganic carbon sources. Culture biomass was not allowed to increase beyond levels that would significantly affect the dissolved carbon system in the closed culture s. In experiments with Skeletonema costatum and Emiliania huxleyi, inc reasing CO2 concentrations led to increased carbon isotope discriminat ion (resulting in organic matter progressively depleted in deltaC-13C, i.e., a greater, more negative epsilon(p)). Epsilon(p) values for E. huxleyi were 8-10 parts per thousand less than for S. costatum under i dentical conditions. For the S. costatum cultures, there was nearly a 20%. range in [CO2aq]-dependent epsilon(p). The effect was nonlinear w ith a leveling off at high [CO2aq]. Over a pH range of 7.5-8.3 but at a constant [CO2aq] there was a variation in carbon isotope fractionati on by S. costatum of about 9 parts per thousand with a minimum at pH 7 .8-7.9. There was a temperature effect of -8 parts per thousand on fra ctionation even after equilibrium temperature dependency of deltaC-13 of CO2aq was taken into account. No growth rate effect was found for S . costatum over a modest range of growth rates. Culture experiments us ed to determine the carbon isotope fractionation by phytoplankton spec ies must be conducted under well-defined conditions of temperature, pH , and CO2 concentrations. Hindcasts of ancient atmospheric pCO2 from m easurements of deltaC-13 of organic carbon in marine sediments will re quire careful calibration because of the variety of possible factors t hat influence deltaC-13org.