CHRONIC-RENAL-FAILURE AND SECONDARY HYPER PARATHYROIDISM IN RATS - BIOCHEMICAL AND HISTOLOGICAL PARAMETERS

Chronic renal failure (CRF) in rats (surgical nephrectomy, 5/6) as wel l as its derived bone lesions have been studied. Eighty-five male Wist ar rats were used, to which chronic renal failure was induced in 1 or 2 surgical times, the parameters of renal function in basal conditions and at different times after surgery being determined. With the metho d used chronic renal failure is induced with values of creatinine clea rance 2/3 times lower than the initial ones (p<0.05), which stabilize at the 7th week. On the other hand the parathyroid hormone levels (PTH ) in serum triple (from 125 +/- 44 to 395 +/- 191, p<0.05), and a decr ease in the tubular phosphate reabsorption is produced (p<0.001). In b one histology an increase in resorption and bone formation is observed as well as paratrabecular fibrosis, all of which is compatible with t he histological diagnosis of hyperparathyroidism. The model of surgica l renal insufficiency with ablation of 5/6 of the renal mass, reduces renal function to 1/3 of the initial values after 7 weeks, this proced ure having a 20 % global mortality without differences being observed between the carrying out of nephrectomies in 1 or 2 surgical times. Th is degree of CRF was accompanied by secondary hyperparathyroidism both at the biochemical and histological levels, findings which are of gre at usefulness for future experimental studies.