THE INFLUENCE OF FETAL CALF SERUM ON INTERFERON-GAMMA-INDUCED HLA-DR EXPRESSION ON U937 CELLS

Authors
GAO J FOLGHERA S FIORENTINI S TINTI M VARINACCI C FLAMMINIO G PERONI L CARUSO A
Citation
J. Gao et al., THE INFLUENCE OF FETAL CALF SERUM ON INTERFERON-GAMMA-INDUCED HLA-DR EXPRESSION ON U937 CELLS, Journal of biological regulators and homeostatic agents, 7(4), 1993, pp. 115-120
Citations number
NO
Categorie Soggetti
Biology,"Medicine, Research & Experimental
Journal title
Journal of biological regulators and homeostatic agents → ACNP
ISSN journal
0393974X
Volume
7
Issue
4
Year of publication
1993
Pages
115 - 120
Database
ISI
SICI code
0393-974X(1993)7:4<115:TIOFCS>2.0.ZU;2-Z
Abstract
The induction of HLA-DR antigen expression on U937 cells by interferon -gamma (IFN-gamma) is positively influenced by amount of fetal calf se rum (FCS) added to the tissue culture medium. A transient alkalinizati on of FCS before its addition to the medium, dramatically decreased th e immunomodulating activity of IFN-gamma. FCS was also found to be a d ose-dependent enhancer of the IFN-gamma-induced 2',5'-oligoadenylate ( 2-5A) synthetase production. Our findings suggest the need for a serum factor(s), labile at basic pH values, to support at least two of the multiple IFN-gamma activities.