Wl. Albritton et L. Chui, SEQUENCE-ANALYSIS OF 2 DELETION MUTANTS IN THE HISC GENE OF SALMONELLA-TYPHIMURIUM, Canadian journal of microbiology, 40(2), 1994, pp. 158-160
Limited sequencing of the terminal region of the hisC gene in two dele
tion mutants involving the hisC gene of Salmonella typhimurium was car
ried out after polymerase chain reaction amplification of the appropri
ate region, using oligonucleotide primers selected from the published
sequence of the histidine operon from this organism. his2648 was shown
to have a 34 base pair deletion in the terminal region of the hisC ge
ne between the P2 promoter and the Shine-Delgamo sequence of the hisB
gene. hisHB22 was shown to have a 1.4 kilobase deletion extending from
the TGA termination codon of the hisC gene to the middle of the hisH
gene. The sequence data were consistent with previous genetic and phen
otypic characterization of these strains.