D. Pavingerova et al., TRANSFORMATION OF RHODODENDRON SPP USING AGROBACTERIUM-TUMEFACIENS WITH A GUS-INTRON CHIMERIC GENE, PLANT SCI, 122(2), 1997, pp. 165-171
The five Rhododendron cultivars, 'America', 'Catawbiense grandiflorum
roseum', 'Madame Carvalho', 'Mars' and 'Nova Zembla' were used for tra
nsformation by Agrobacterium tumefaciens carrying T-DNA with the gusA
gene encoding beta-glucuronidase (GUS) gene and the neomycin phosphotr
ansferase II gene as a selectable marker gene. The GUS reporter gene w
as successfully transferred into all five cultivars as indicated by fl
uorimetric staining, polymerase chain reaction (PCR) and Southern blot
analysis. Some primary transformants appeared to be chimeric as both
GUS expression and GUS nucleotide sequences were lost during vegetativ
e propagation. (C) 1997 Elsevier Science Ireland Ltd.