K. Nakajima et M. Sorimachi, BIOLOGICAL AND PHYSICOCHEMICAL PROPERTIES OF THE IRIDOVIRUS ISOLATED FROM CULTURED RED-SEA BREAM, PAGRUS-MAJOR, Gyobyo kenkyu, 29(1), 1994, pp. 29-33
Eleven fish cell lines were compared for susceptibility to red sea bre
am iridovirus (RSIV). Cytopathic effect was produced in BF-2, CHSE-214
, FHM, JSKG, KRE-3, RTG-2 and YTF cell lines among the cell lines exam
ined. The titers of the virus on BF-2 and KRE-3 were higher than those
on the other cell lines. The pretreatment of BF-2 and KRE-3 cells wit
h polyethylene glycol (PEG) or addition of PEG to the sample increased
the virus titers. RSIV replicated at 15-degrees-C, 20-degrees-C, 25-d
egrees-C and 30-degrees-C but not at 37-degrees-C. The suitable temper
ature for viral growth was 20-degrees-C or 25-degrees-C. The virus was
sensitive to acid (pH 3), chloroform and ether and unstable to heat b
ut stable to ultrasonic treatment and repeated freezing and thawing. T
reatment with 5-iodo-2-deoxyuridine (IUdR) reduced the titers of the v
irus.