Y. Tabuchi et K. Furuhama, INHIBITORY EFFECT OF DS-4574, A MAST-CELL STABILIZER WITH PEPTIDOLEUKOTRIENE RECEPTOR ANTAGONISM, ON GASTRIC-ACID SECRETION IN RATS, European journal of pharmacology, 255(1-3), 1994, pp. 229-234
We examined the inhibitory effect of DS-4574 yl)[1,3,4]thiadiazolo[3,2
-a]-1,2,3-triazolo[4,5-d] pyrimidin-9(3H)-one), a mast cell stabilizer
with peptidoleukotriene receptor antagonism, on gastric acid secretio
n stimulated by several secretagogues in rats. In anesthetized rats wi
th acute gastric fistulas, DS-4574 (50 mg/kg, intraduodenal) significa
ntly inhibited gastric acid secretion induced by both carbachol (50 mu
g/kg, s.c.) and pentagastrin (75 mu g/kg, s.c.), but not by histamine
(2.5 mg/kg, s.c.). In unanesthetized pylorus-ligated rats, DS-4574 (1
0 and 25 mg/kg, intraduodenal) markedly suppressed increases in gastri
c acid output and histamine leakage into the gastric juice produced by
carbachol (0.1 mg/kg, s.c.) or pentagastrin (1 mg/kg, s.c.). When the
relationship between acid output and histamine leakage elicited by ca
rbachol and pentagastrin was assessed, there was a close correlation (
r = 0.84) that was highly significant (P < 0.01). In the in vitro stud
y with rat gastric tissues, DS-4574 (10(-7)-10(-5) M) had no effect on
the K+-dependent ATPase activity or on aminopyrine uptake into mucosa
l preparations containing parietal cells stimulated by carbachol (10(-
5) M), histamine (10(-4) M), or dibutyryl-cyclic AMP (10(-3) M). These
results suggest that the effect of DS-4574 may be mediated by inhibit
ion of endogenous histamine from histamine-storing cells in the stomac
h.