USE OF SIGNAL-DISTINGUISHABLE PROBES IN DIFFERENTIAL OR SEQUENTIAL AUTORADIOGRAPHY IN HYBRIDIZATION ANALYSIS

Authors
AU LC CHANG KJ SHIH CM TEH GW
Citation
Lc. Au et al., USE OF SIGNAL-DISTINGUISHABLE PROBES IN DIFFERENTIAL OR SEQUENTIAL AUTORADIOGRAPHY IN HYBRIDIZATION ANALYSIS, BioTechniques, 16(4), 1994, pp. 680-683
Citations number
7
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
16
Issue
4
Year of publication
1994
Pages
680 - 683
Database
ISI
SICI code
0736-6205(1994)16:4<680:UOSPID>2.0.ZU;2-E
Abstract
To avoid the time-consuming reprobing process in hybridization analysi s, signal distinguishable probes (P-32, S-35 or antigenic hapten-label ed DNA) can be added to the same hybridization mixture. After hybridiz ation an unambiguous result can be obtained by differential or sequent ial autoradiography.