COENZYME PRODUCTION USING IMMOBILIZED ENZYMES .2. FACTORS AFFECTING THE CONTINUOUS PRODUCTION OF NADP(+)

The effects of certain factors on the continuous production of NADP(+) with immobilized NAD(+) kinase were studied. In a column reactor the maximum NADP(+) productivity was experienced at pH 8.0. The presence o f 10-12 mM Mg2+ ion appeared to be optimum for maximum productivity. K + ion at 1-5 mM behaved as an activator. Mn2+ and Zn2+ ions proved eff ective activators of the immobilized NAD(+) kinase. Systems of up to t hree or four columns were most effective in NADP(+) production.