RECOVERY OF ISOAMYLASE FROM PSEUDOMONAS-AMYLODERAMOSA BY ADSORPTION-ELUTION ON RAW STARCH

The isoamylase from Pseudomonas amyloderamosa WU2130 was adsorbed onto raw starch, and the adsorbed enzyme was eluted by maltose in 50 mM ac etate buffer. The adsorption of isoamylase to adsorbent was affected b y the sour ces of raw starch, temperature, and pH, whereas the tempera ture and elution time had no obvious effects on the elution of adsorbe d isoamylase. Raw starch could be used repeatedly on the adsorption-el ution cycle,vith good reproducibility. About 52.5% of isoamylase was r ecovered, and the eluted enzyme had a specific activity of 72,828 U mg (-1) protein with a purification of 13.3-fold. These results suggest t hat the law starch adsorption-elution technique has a great potential in recovery of isoamylase from culture broths.