RAPID DEVELOPMENT OF TOLERANCE TO MORPHINE IN THE FORMALIN TEST

PREVIOUS reports have suggested that tolerance to the antinociceptive effects of morphine does not develop in the formalin test. To re-exami ne these findings, morphine was administered via multiple injections o r continuous infusion from 0 to 4 days prior to testing with either th e formalin or tail-flick test. Following twice daily injections (5.0 m g kg(-1) s.c.), significant tolerance developed within 2 days with the formalin test but not until day 4 with the tail-flick test. Significa nt tolerance was noted with both tests 4 days following the implantati on of osmotic minipumps (10 mg kg(-1) day(-1), s.c.). We conclude that in mice, tolerance to the analgesic effects of morphine develops rapi dly with the formalin test.