MOLECULAR-CLONING OF THE GENE ENCODING A HIGHLY EXPRESSED PROTEIN IN SFL1 GENE-DISRUPTED FLOCCULATING YEAST

We identified a yeast gene encoding Flocculent Specific Protein (FSP) produced excessively in the SFL1 gene-disrupted flocculent strain. The sequenced gene encodes a 430 amino acid protein and is mainly compose d of multiple repeats of Ser-Asn-Asn-X-Asp-Ser-Tyr-Gly. The FSP gene d isruption of the flocculent strain decreased the degree of flocculatio n, so FSP may be one factor concerned with yeast flocculation. A gene database search indicated that the FSP gene is identical with the DDR4 8 gene.