PREPARATION AND PURIFICATION OF BETA-AMYLOID(1-43) VIA SOLUBLE, AMIDEBACKBONE PROTECTED INTERMEDIATES

We report a new synthetic strategy for the preparation of the amyloido genic protein fragment beta A4, through an easily purified (and charac terized) intermediate of high aqueous solubility. This novel intermedi ate consists of beta A4(1-43) with backbone amide protection, provided by the N-(2-hydroxy-4-methoxybenzyl) group, on residues Phe(20) and G ly(25,29,33,38). The new strategy enabled derivatized beta A4(1-43) to be isolated and purified by standard reverse-phase HPLC procedures an d characterized by electrospray mass spectrometry (ESMS).