THE ULTRASTRUCTURE OF SEED COAT DEVELOPMENT IN RANUNCULUS-SCELERATUS

Seed-coat development in Ranunculus sceleratus L. has been studied by electron microscopy. Three layers developed from the single integument . The outer epidermis consisted of elongated and flattened cells that were always well attached to each other. The cells were characterized by thin walls, the presence of chloroplasts and small vacuoles. The ce lls of the middle layer were originally closely packed. Gradually, ext ensive intercellular spaces were formed. The cells of the inner epider mis elongated initially, until they became cubic and developed a thick wall with numerous wall ingrowths at the side bordering the nucellus. Thus, they give rise to a mechanical layer protecting the inner part of the seed. The elongation of cells, the thickening of cell walls, th e formation of wall ridges, and the formation of intercellular spaces each coincided with characteristic configurations of microtubules. Pla smodesmata were originally found between all cells of the integument b ut their number decreased drastically during development, especially b etween the three developing seed-coat layers. Well-differentiated chlo ro-amyloplasts, present in all cells of the developing seed-coat, poin ted to autotrophy during development. Maturation eventually led to the disappearance of cytoplasm in all cells, the compression of the cells of the outer epidermis and middle layers, and the formation of a mech anical layer from the inner epidermis.