WHEY AND BETA-LACTOGLOBULIN .2. MILK BY-PRODUCTS WHICH CAN REPLACE FETAL CALF SERUM IN MOUSE HYBRIDOMA CELL-CULTURE

In previous papers we showed that bovine whey allows short-term hybrid oma cell culture and that for long-term culture it was necessary to ad d 1% fetal calf serum. In this paper we are demonstrating firstly, how to substitute 1% fetal calf serum by 1% horse serum and show that the mixture, 9% bovine whey + 1% horse serum, gives similar cell growth r esults and antibody production results; secondly, we are demonstrating that this mixture, less expensive, could be lyophilized which allows to envisage industrially processed preparations; and finally, how in o rder to obtain a substitute which is better defined and still less exp ensive than fetal calf serum, one can replace bovine whey by its major protein, beta-lactoglobulin, which has also been complemented with 1% horse serum.