IMPROVED METHOD FOR DNA EXTRACTION FROM VITIS-VINIFERA

Authors
STEENKAMP J WIID I LOURENS A VANHELDEN P
Citation
J. Steenkamp et al., IMPROVED METHOD FOR DNA EXTRACTION FROM VITIS-VINIFERA, American journal of enology and viticulture, 45(1), 1994, pp. 102-106
Citations number
23
Categorie Soggetti
Food Science & Tenology",Agriculture,"Biothechnology & Applied Migrobiology
Journal title
American journal of enology and viticulture → ACNP
ISSN journal
00029254
Volume
45
Issue
1
Year of publication
1994
Pages
102 - 106
Database
ISI
SICI code
0002-9254(1994)45:1<102:IMFDEF>2.0.ZU;2-3
Abstract
An inexpensive and high yielding procedure is described for the isolat ion of DNA from small quantities of leaves or roots from Vitis vinifer a. The buffer capacity of the extraction solution was found to be the single most important factor in the isolation of the DNA. Different de tergent (CTAB) concentrations have an effect on the efficiency of clea vage by restriction endonucleases. The quality of the DNA was checked by fingerprint analysis after digestion with HaeIII and HinfI restrict ion enzymes and probing with the multilocus pV47-2 probe. Differences between Vitis vinifera cultivars were detected.