OPTIMIZATION OF AMINO-ACID SUPPLEMENTS FOR HETEROLOGOUS PROTEIN SECRETION IN SACCHAROMYCES-CEREVISIAE

Authors
WITTRUP KD BENIG V
Citation
Kd. Wittrup et V. Benig, OPTIMIZATION OF AMINO-ACID SUPPLEMENTS FOR HETEROLOGOUS PROTEIN SECRETION IN SACCHAROMYCES-CEREVISIAE, Biotechnology techniques, 8(3), 1994, pp. 161-166
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
3
Year of publication
1994
Pages
161 - 166
Database
ISI
SICI code
0951-208X(1994)8:3<161:OOASFH>2.0.ZU;2-A
Abstract
The two most commonly used amino acid media supplements do not improve the specific rate of secretion of Schizosaccharomyces pombe acid phos phatase in S. cerevisiae relative to unsupplemented Synthetic Dextrose (SD) media. New amino acid supplement formulations have been obtained which produce an 8-fold increase in total phosphatase secretion, and a 70% increase in specific phosphatase secretion.