RAPID PURIFICATION OF ALPHA-LYTIC PROTEASE AND ITS MUTANTS FROM SMALLCULTURES OF RECOMBINANT ESCHERICHIA-COLI

Authors
HAGGETT KD GRAHAM LD WHITTAKER RG
Citation
Kd. Haggett et al., RAPID PURIFICATION OF ALPHA-LYTIC PROTEASE AND ITS MUTANTS FROM SMALLCULTURES OF RECOMBINANT ESCHERICHIA-COLI, Biotechnology techniques, 8(3), 1994, pp. 203-208
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
3
Year of publication
1994
Pages
203 - 208
Database
ISI
SICI code
0951-208X(1994)8:3<203:RPOAPA>2.0.ZU;2-X
Abstract
A single-step procedure is described for purifying alpha-lytic proteas e and its mutants on a small scale (approx. 5mg) from cell-free supern atants of recombinant E. coli. Although free of non-enzyme protein, th ese preparations often contain fragments that arise from enzymatic sel f-digestion. Two strong cation exchange matrices are shown to vary gre atly in their ability to separate such degradation products from intac t enzyme.