STRUCTURE AND EXPRESSION IN BREAST-TUMORS OF HUMAN TIMP-3, A NEW MEMBER OF THE METALLOPROTEINASE INHIBITOR FAMILY

Citation
Ja. Uria et al., STRUCTURE AND EXPRESSION IN BREAST-TUMORS OF HUMAN TIMP-3, A NEW MEMBER OF THE METALLOPROTEINASE INHIBITOR FAMILY, Cancer research, 54(8), 1994, pp. 2091-2094
Citations number
20
Categorie Soggetti
Oncology
Journal title
ISSN journal
00085472
Volume
54
Issue
8
Year of publication
1994
Pages
2091 - 2094
Database
ISI
SICI code
0008-5472(1994)54:8<2091:SAEIBO>2.0.ZU;2-V
Abstract
A new member of the metalloproteinase inhibitor family of proteins has been cloned from a complementary DNA library derived from a human bre ast tumor. The isolated complementary DNA contains an open reading fra me 633 base pairs long, encoding a polypeptide of 211 amino acids, whi ch has been called tissue inhibitor of metalloproteinase 3 (TIMP-3). T his protein displays low sequence similarity to the previously known h uman TIMPs but shows a high degree of similarity with chicken inhibito r of metalloproteinase 3, a recently described metalloproteinase inhib itor stimulated during oncogenic transformation of chicken fibroblasts and with the ability to promote some phenotypic properties of transfo rmed cells. Northern blot analysis of RNA from human tissues revealed that the TIMP-3 gene is expressed in placenta and uterus but not in li ver and ovary. In addition, TIMP-3 transcripts were detected in all br east carcinomas examined. On the basis of these expression data in bre ast tumors, together with its high degree of structural homology with chicken inhibitor of metalloproteinase 3, a possible role for human TI MP-3 in the regulation of connective tissue turnover and remodeling is proposed.