Shoot proliferation of Japanese plum yew [Cephalotaxus harringtonia (F
orbes) K. Koch] derived either from a seedling or from a mature clone
was achieved in a medium based on Murashige and Skoog salts supplement
ed with vitamins and casein hydrolysate in the presence of 10% (v/v) c
oconut water. Rooting was unsuccessful from microcuttings proliferated
on agar-based medium, but, when cultured in liquid medium on membrane
rafts, detached shoots rooted under mist in greenhouse conditions wit
h or without auxin treatments. Rooted microcuttings successfully accli
mated to greenhouse conditions.