INVESTIGATION OF GLIADIN BINDING TO DIFFERENT SELECTED PROTEINS USINGA BIOTIN-STREPTAVIDIN SYSTEM

Using a biotin streptavidin system 12 out of 19 different test protein s were found to bind to gliadin, with casein, fibrinogen, and fibronec tin showing the strongest association. Binding was demonstrable using different gliadin subfractions and enzymatic digests. There was no con sistent effect of several carbohydrates on binding. Binding of most pr oteins to gliadin was diminished slightly by removal of Ca2+ and stron gly by dioxane, ethylene glycol, Triton X-100, and Tween 20. The resul ts suggest a non-selective association between gliadin and the protein s investigated, mainly due to hydrophobic interactions, which might be of significance in intestinal pathobiochemistry, e. g. disease.