The uptake of phosphate by benthic communities, dominated by living al
gae, previously exposed to different levels of external nutrient loadi
ng, exhibited first-order kinetics with respect to the intracellular P
-deficit. This deficit is the difference between the maximum and the a
ctual intracellular P-concentration. The maximum storage capacity of P
per unit of dry weight was positively correlated to the level of exte
rnal nutrient loading, whereas the phosphate uptake rate constant was
negatively correlated. The observed internal P concentrations in the b
enthic layer of test ditches over a period of two and a half years, in
dicated a slight decrease towards a minimum value in a ditch with a lo
w external P-input. In a medium loaded ditch the internal P-concentrat
ion did not change significantly. In a high loaded ditch increasing in
ternal P-concentrations over time were observed, towards P-saturation
of the benthic community.