HEPATIC LIPASE INDUCES THE FORMATION OF PRE-BETA(1) HIGH-DENSITY-LIPOPROTEIN (HDL) FROM TRIACYLGLYCEROL-RICH HDL(2) - A STUDY COMPARING LIVER PERFUSION TO IN-VITRO INCUBATION WITH LIPASES
A. Barrans et al., HEPATIC LIPASE INDUCES THE FORMATION OF PRE-BETA(1) HIGH-DENSITY-LIPOPROTEIN (HDL) FROM TRIACYLGLYCEROL-RICH HDL(2) - A STUDY COMPARING LIVER PERFUSION TO IN-VITRO INCUBATION WITH LIPASES, The Journal of biological chemistry, 269(15), 1994, pp. 11572-11577
High density lipoprotein subfractions with a pre-beta migration play a
key role in the reverse cholesterol transport. The origin of these pa
rticles is not yet clearly defined. We propose to verify a possible or
igin of these particles during the catabolism of high density lipoprot
ein 2 (HDL2) by hepatic lipase using two different models. A rat liver
perfusion of native human HDL2 in the presence of heparin induced, af
ter 30 min, the formation of the pre-beta1, HDL subspecies. Human HDL2
enriched with triacylglycerols, perfused in the same conditions, led
after 15 min to an enhanced production of this pre-beta1 HDL populatio
n, as compared with the results obtained with native HDL2. A reduction
of the alpha-HDL2 fraction was also evident. After perfusion, a simil
ar formation of pre-beta1 HDL from triacylglycerol-rich HDL2 was obser
ved in absence of heparin. When these HDL2 were incubated in vitro for
120 min at 37-degrees-C in the presence of partially purified rat hep
atic lipase, the appearance of pre-beta1 HDL was again found and assoc
iated with a decrease in size of the remaining alpha-HDL subfractions
as compared with original HDL2. On the contrary, the incubation of the
same HDL2 with snake venom phospholipase A2 produced no pre-beta HDL.
These results evidence the role of the triacylglycerol lipase activit
y of hepatic lipase in the formation of pre-beta1 HDL from triacylglyc
erol-rich HDL2.