PHILADELPHIA-CHROMOSOME AS THE SOLE ABNORMALITY AND P210 BCR-ABL CHIMERIC PROTEIN EXPRESSION IN AN EPSTEIN-BARR VIRUS-TRANSFORMED B-CELL LINE FROM A PATIENT WITH CHRONIC MYELOID-LEUKEMIA
S. Knuutila et al., PHILADELPHIA-CHROMOSOME AS THE SOLE ABNORMALITY AND P210 BCR-ABL CHIMERIC PROTEIN EXPRESSION IN AN EPSTEIN-BARR VIRUS-TRANSFORMED B-CELL LINE FROM A PATIENT WITH CHRONIC MYELOID-LEUKEMIA, Acta haematologica, 90(4), 1993, pp. 190-194
A 'B' cell line, originating from a patient with chronic myeloid leuke
mia and containing the Philadelphia chromosome, was established after
Epstein-Barr virus transformation. The Philadelphia chromosome was the
sole chromosomal abnormality in this line, designated as PhB1 cell li
ne. In DNA hybridization studies we detected rearrangements in the bcr
gene and in the immunoglobulin heavy chain joining region. The phenot
ypes of the cells were typical of mature B cells expressing antigens C
D19, CD20, CD22, CD23, CD39, HLADR, IgM, and kappa. The expression of
the 210 bcr-abl chimeric protein was detected by means of an immunopre
cipitate assay.