IMMUNIZATION OF GUINEA-PIGS WITH TREPONEMA-PALLIDUM RECOMBINANT ANTIGENS REVEALS THE PRESENCE OF NOVEL EPITOPES

Authors
WICHER K ABBRUSCATO F WICHER V SCHOULS L
Citation
K. Wicher et al., IMMUNIZATION OF GUINEA-PIGS WITH TREPONEMA-PALLIDUM RECOMBINANT ANTIGENS REVEALS THE PRESENCE OF NOVEL EPITOPES, International archives of allergy and immunology, 103(4), 1994, pp. 396-399
Citations number
4
Categorie Soggetti
Allergy,Immunology
Journal title
International archives of allergy and immunology → ACNP
ISSN journal
10182438
Volume
103
Issue
4
Year of publication
1994
Pages
396 - 399
Database
ISI
SICI code
1018-2438(1994)103:4<396:IOGWTR>2.0.ZU;2-K
Abstract
The DNA technology employed in the construction and purification of re combinant antigens has the potential of creating epitopes with specifi cities other than those of native antigens. Such a phenomenon has been observed when guinea pigs were immunized with Treponema pallidum reco mbinant antigens, TmpA and TmpB, expressed in Escherichia coli K12. Ad sorption of the immune sera with E. coil K12 and T. pallidum revealed the presence of antibodies directed against epitopes not present or ex posed in the native antigens of the organisms from which the DNA has b een cloned.