Clonal cultures of Prorocentrum hoffmannianum Faust (clone 882a) were
grown under optimal environmental conditions for maximal okadaic acid
production. The environmental conditions of 25-degrees-C and 86 mumol
photon m-2 s-1 were used to cultivate P. hoffmannianum in a semi-conti
nuous 36-L culture vessel with continuous cell suspension and pH contr
ol. Using these conditions, a 3-fold increase in harvestable biomass a
nd okadaic acid content was observed when compared to batch culture te
chniques.