DETERMINATION OF GLYCOSYLATION SITES IN O-LINKED GLYCOPEPTIDES - A SENSITIVE MASS-SPECTROMETRIC PROTOCOL

Authors
RADEMAKER GJ HAVERKAMP J THOMASOATES J
Citation
Gj. Rademaker et al., DETERMINATION OF GLYCOSYLATION SITES IN O-LINKED GLYCOPEPTIDES - A SENSITIVE MASS-SPECTROMETRIC PROTOCOL, Organic mass spectrometry, 28(12), 1993, pp. 1536-1541
Citations number
25
Categorie Soggetti
Chemistry Inorganic & Nuclear",Spectroscopy
Journal title
Organic mass spectrometry → ACNP
ISSN journal
0030493X
Volume
28
Issue
12
Year of publication
1993
Pages
1536 - 1541
Database
ISI
SICI code
0030-493X(1993)28:12<1536:DOGSIO>2.0.ZU;2-2
Abstract
A sensitive mass spectrometric method for the specific identification of O-glycosylation sites in glycopeptides is presented. Reductive beta -elimination liberates a peptide containing a specifically modified am ino acid at the former site of carbohydrate attachment. Mass spectrome try is used to determine the sequence of the modified peptide, in whic h the position of the modified amino acid marks the site of O-glycosyl ation. The method also provides information on the carbohydrate chain and is applicable at the low nanomolar level.