J. Przyborowski et K. Niemirowiczszczytt, MAIN FACTORS AFFECTING CUCUMBER (CUCUMIS-SATIVUS L) HAPLOID EMBRYO DEVELOPMENT AND HAPLOID PLANT CHARACTERISTICS, Plant breeding, 112(1), 1994, pp. 70-75
This report gives the first detailed analysis of cucumber haploid plan
ts and the first evaluation of genotype and season effects on haploid
embryo development. Female flowers of four Fl varieties and two inbred
lines were pollinated with 300 Gy-treated pollen and three-week old e
mbryos were cultured in vitro on E20A medium. The variety 'Polan' prod
uced the greatest number of embryos (1.34 per 100 seeds), 51 % of whic
h were capable of further development. The number of embryos able to d
evelop was four times higher in summer than in spring. All the plants
obtained, except one, were haploids or aneuhaploids. Haploid plants gr
ew slowly and were sterile, although they occasionally set seedless fr
uits. The length of the stems, leaves and guard cells was smaller than
those of diploids.