PROSTAGLANDIN-E (PGE) AND INTERLEUKIN-1-BETA (IL-1-BETA) LEVELS IN GINGIVAL CREVICULAR FLUID DURING HUMAN ORTHODONTIC TOOTH MOVEMENT

The purpose of this study was to examine gingival crevicular fluid (GC F) levels of two potent bone resorbing mediators, prostaglandin E (PGE ) and interleukin-1beta (IL-1beta), during human orthodontic tooth mov ement. The study included 10 patients, each having one treatment tooth undergoing orthodontic movement and a contralateral control tooth. Th e GCF was sampled at control sites and treatment (compression) sites b efore activation and at 1, 24, 48, and 168 hours. Prevention of plaque -induced inflammation allowed this study to focus on the dynamics of m echanically stimulated PGE and IL-1beta GCF levels. The PGE and IL-1be ta levels were determined with radioimmunoassay. At 1 and 24 hours, me an GCF IL-1beta levels were significantly elevated at treatment teeth (8.9 +/- 2.0 and 19.2 +/- 6.0 pg, respectively) compared with control teeth (2.0 +/- 1.1 pg, p = 0.0049, and 2.9 - 1.0 pg, p = 0.0209, respe ctively). The GCF levels of PGE for the treatment teeth were significa ntly higher at 24 and 48 hours (108.9 +/- 11.9 and 97.9 +/- 7.3 pg) th an the control teeth (61.8 +/- 7.2 pg, p = 0.0071, and 70.8 +/- 7.4 pg , p = 0.0021, respectively). The GCF levels of PGE and IL-1beta remain ed at baseline levels throughout the study for the control teeth, wher eas significant elevations from baseline in GCF IL-1beta (24 hours) an d PGE levels (24 and 48 hours) were observed over time in the treatmen t teeth (p less-than-or-equal-to 0.05). These results demonstrate that bone-resorbing PGE and IL-1beta produced within the periodontium are detectable in GCF during the early phases of tooth movement and return to baseline within 7 days.