DETECTION OF HUMAN CYTOMEGALOVIRUS GENOME IN UTERUS TISSUE

Citation
T. Furukawa et al., DETECTION OF HUMAN CYTOMEGALOVIRUS GENOME IN UTERUS TISSUE, Archives of virology, 135(3-4), 1994, pp. 265-277
Citations number
38
Categorie Soggetti
Virology
Journal title
ISSN journal
03048608
Volume
135
Issue
3-4
Year of publication
1994
Pages
265 - 277
Database
ISI
SICI code
0304-8608(1994)135:3-4<265:DOHCGI>2.0.ZU;2-B
Abstract
To analyze persistent infection by human cytomegalovirus (HCMV) in viv o, specimens obtained from various sources and autopsied organs were e xamined for the presence of HCMV DNA, mRNA transcripts and antigens by polymerase chain reaction, in situ hybridization and immunostaining. The HCMV genome was detected in lung, liver, kidney, and blood vessels at an average positive rate of 15%. The highest PCR-positive rate was observed with cervical smears. Subsequent examination of uterus tissu es from patients with myoma revealed HCMV transcripts and antigens in glandular epithelial cells, leukocytes, endothelial cells, and others, indicating productive HCMV infection of cervical tissue.