PHYSIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF HEPATITIS-A VIRUS NUCLEOCAPSIDS EXPRESSED IN A VACCINIA VIRUS T7/EMCV SYSTEM - BRIEF REPORT/

Authors
ZHU NL LI GD WANG Y
Citation
Nl. Zhu et al., PHYSIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF HEPATITIS-A VIRUS NUCLEOCAPSIDS EXPRESSED IN A VACCINIA VIRUS T7/EMCV SYSTEM - BRIEF REPORT/, Archives of virology, 135(3-4), 1994, pp. 443-449
Citations number
14
Categorie Soggetti
Virology
Journal title
Archives of virology → ACNP
ISSN journal
03048608
Volume
135
Issue
3-4
Year of publication
1994
Pages
443 - 449
Database
ISI
SICI code
0304-8608(1994)135:3-4<443:PAICOH>2.0.ZU;2-4
Abstract
Expression of the entire open reading frame of hepatitis A virus was a chieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antig ens were characterized as particles with buoyant densities and sedimen tation coefficients typical of emtpy capsids and pentamers of HAV. Thi s was further confirmed by electron microscopy. All capsid proteins we re determined as components of the particles which raised neutralizing antibodies in an immunized rabbit.