SIMPLE METHOD FOR EXTRACTING PLASMID DNA FROM LACTIC-ACID BACTERIA

Authors
FRERE J
Citation
J. Frere, SIMPLE METHOD FOR EXTRACTING PLASMID DNA FROM LACTIC-ACID BACTERIA, Letters in applied microbiology, 18(4), 1994, pp. 227-229
Citations number
8
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
18
Issue
4
Year of publication
1994
Pages
227 - 229
Database
ISI
SICI code
0266-8254(1994)18:4<227:SMFEPD>2.0.ZU;2-T
Abstract
Rapid screening and large-scale plasmid DNA isolation procedures are d escribed for lactic acid bacteria, using glass beads to break cells. T he rapid screening procedure allows one to obtain plasmid DNA pellets in less than 1 h. This method has been successfully tested on various bacteria from the genera Lactococcus, Leuconostoc, Lactobacillus, Pedi ococcus, Streptococcus, Enterococcus and Propionibacterium. This proce dure yields plasmid DNA with minor chromosomal and plasmid DNA-degrade d form contaminations.