ADENOSINE PRODUCTION BY A MUTANT OF BACIL LUS-SUBTILIS RESISTANT TO ADENINE ANALOGS

A mutant resistant to adenine analogs was derived from an adenosine pr oducer, Bacillus subtilis A 3-5, which had xanthine auxotrophy, 8-azag uanine resistance, and sulfaguanidine resistance. Selection during bre eding was done by screening for resistance to 2-thioadenine sulfate (0 .5 mg/ml), resistance to 8-azaadenine (1 mg/ml), and resistance to 6-m ethylaminopurine (1 mg/ml), in that order. The strain obtained, No.43- 35, produced more adenosine than the parent strain. For increased prod uction of adenosine, the effects of the cultural conditions were exami ned. Strain No. 43-35 produced 24 g/l adenosine from 130 g/l glucose i n a glass jar fermenter with a working volume of 300 ml at the best cu ltural conditions found.