D-FRUCTOSE SENSING ELECTRODE BASED ON ELECTRON-TRANSFER OF D-FRUCTOSEDEHYDROGENASE AT COLLOIDAL GOLD-ENZYME MODIFIED ELECTRODE

A complex of colloidal gold (Au) particles and D-fructose dehydrogenas e was prepared. A glassy carbon (GC) electrode was modified with the c omplex. The anodic current response to D-fructose was measured by usin g the GC electrode modified with the complex at +0.5 V (vs. Ag/AgCl). Response time was within 15 s. No current response was observed in the absence of enzyme, so that the increased oxidation current was confir med to be caused by the electron transfer from enzyme to electrode. Li near response range of the electrode was up to 0.5 mM fructose concent ration. Fructose concentrations of soft drinks which were determined u sing the electrode agreed well with the results of the F-kit.