A. Scaltsoyiannes et al., MICROPROPAGATION OF THE PINE HYBRID PINUS-BRUTIA (TEN) X PINUS-HALEPENSIS (MILL) BY CULTURING FASCICLE SHOOTS, Annales des Sciences Forestieres, 51(2), 1994, pp. 175-182
Fascicle shoots proved to be an ideal plant material for micropropagat
ion of the pine hybrid P brutia (Ten) x P halepensis (Mill). This coul
d be possibly attributed to their morphological and physiological stat
e. Induced fascicle shoots of 4-yr-old seedlings were used as explants
. Their induction was achieved by spraying once with the new herbicide
Arsenal (1 000 mg l-1). First, explants were elongated in vitro on LP
medium and then transferred to the multiplication stage. Multiplicati
on was accomplished by decapitating, quick-dipping in 0.22 mM BA and i
noculating the explants on BIMI medium. On the induced first-generatio
n shoots the same procedure was applied, in order to obtain second-gen
eration shoots and then these were proceeded to the rooting and acclim
atization stages. In particular, when microcuttings were pretreated wi
th 2.46 muM IBA + 2.7 muM NAA + 0.65% agar w/v + 1.5% sucrose w/v for
7 d and then transferred to greenhouse conditions, a good root system
was developed within an 8-12-week period. A large variation in rootabi
lity was noted between clones. The above method may be proved to be ef
ficient for clones that exhibit high rooting ability.